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tfiib  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc tfiib
    Tfiib, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tfiib/pm40739040-290-0-6
    Average 86 stars, based on 1 article reviews
    tfiib - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: Intracellular Cytomatrix, Immobilized Biocatalysis, Matrix Micromechanics and The Warburg Effect: Entanglement of Two Age-Old Mysteries of the Normal and Malignant Cell
    Article Snippet: Reagents were obtained from the following sources: antibodies to mTOR (2983), 4E-BP1 (9644), PDI (3501), FoxA1 (53528), GATA-6 (5851), TFIIB (4169), CHOP (2895), DDX6/RCK (9407), FUBP 1/2 (13398), PTBP1/2 (57246), hnRNPK (9081), LAMP1 (9091), Caveolin-1 (3267), AIF (5318), Annexin (32934), Rac1/2/3 (2465), RCAS1 (12290), EEA1 (3288), Rab5 (3547), Rab7 (9367), Rab11 (5589), Fibrillarin (2639), Emerin (5430), pan-Keratin (4545), β-Tubulin (2128), β-actin (3700) were purchased from Cell Signaling Technology (Danvers, MA); mTOR kinase inhibitor, TOR-KI, pp242 was purchased from Tocris Bioscience (4257/5).

    Article Title: Cleavage of the RNA polymerase II general transcription factor TFIIB es transcription during stress
    Article Snippet: Diluted samples were quantified by Pierce 660 assay with 0.8% Triton-X.

    Article Title: G-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes.
    Article Snippet: Pyridostatin trihydrochloride (PDS; CAS: T4457) was purchased from Topscience Co., Ltd. Pol II Rpb1 (POLR2A) NTD (D8L4Y) rabbit mAb, Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb, and TFIIB (GTF2B, 2F6A3H4) mouse mAb were purchased from Cell Signaling Technology.

    Article Title: G-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes
    Article Snippet: Pyridostatin trihydrochloride (PDS; CAS: T4457) was purchased from Topscience Co., Ltd. Pol II Rpb1 (POLR2A) NTD (D8L4Y) rabbit mAb, Acetyl-Histone H3 (Lys27) (D5E4) XP Rabbit mAb, and TFIIB (GTF2B, 2F6A3H4) mouse mAb were purchased from Cell Signaling Technology.

    Western Blot:

    Article Title: Multi-proteomic profiling of the varicella-zoster virus–host interface reveals host susceptibilities to severe infection
    Article Snippet: .. TFIIB (western blot (WB) 1:1,000; 4169; Cell Signaling Technology), MPP8 (WB 1:1,500; 16796; Proteintech), ZNF280D (WB 1:1,000; PA5-56410; Invitrogen), NPHP4 (WB 1:3,000; A8934; Abclonal), UBXN7 (IF 1:500; HPA049442; Sigma-Aldrich), IFI16 (WB 1:1,000, IF 1:500; 14970; Cell Signaling Technology), V5-tag rabbit (WB 1:1,000, IF 1:1,000; 13202; Cell Signaling Technology), V5-tag mouse (WB 1:1,000, IF 1:400; R960-25; Invitrogen), HA-tag (WB 1:2,500, IF 1:100; 2367; Cell Signaling Technology), HA-tag-HRP (WB 1:1,000; H6533; Sigma-Aldrich), β-actin-HRP (WB 1:2,500; sc-47778; Santa Cruz), β-tubulin (WB 1:500; 2128; Cell Signaling Technology). .. For WB, secondary antibodies conjugated to HRP detecting rabbit IgG (1:2,500) and mouse IgG (1:5,000) were purchased from Dako and Sigma-Aldrich, respectively.

    Article Title: The RNA polymerase II general transcription factor TFIIB is a target for transcriptome control during cellular stress and viral infection
    Article Snippet: 20-30 μg whole cell lysate was denatured in Laemmli Buffer (Bio-Rad), resolved by SDS-Page, and transferred to PVDF membrane (Bio-Rad) with a Trans-Blot Turbo (Bio-Rad, Mixed Molecular Weight protocol). .. Western blotting was performed with Tris-buffered saline and 0.2% Tween-20 (TBS-T) using PageRuler Prestained protein ladder (10-180 kDa formulation; ThermoFisher) and the following primary and secondary antibodies: TFIIB (clone 2F6A3H4, Cell Signaling-4169, 1:1000), GAPDH (anti-mouse, clone 6C5, Thermofisher-AM4300, 1:5000 or anti-rabbit, clone 14C10, Cell Signaling-2118, 1:1000), p27 (ProteinTech-15086-1-AP, 1:1000), TRIM28 (Cell Signaling-4123, 1:1000), TRIM24 (clone E93TN, Cell Signaling-79030, 1:1000), Vinculin (Abcam-ab91459, 1:1000), FK2 (Enzo-BML-PW8810-0100, 1:1000), NRF2 (ProteinTech-16396-1-AP, 1:000), Caspase-3 (Cell Signaling-9662, 1:1000), Caspase-8 (clone E7, Abcam-ab32397, 1:1000), γH2AX (Bethyl-A300-081A-M, 1:1000), p53 (clone 1C12, Cell Signaling-2524, 1:1000), Goat Anti-Mouse IgG(H+L) Human ads-HRP (Southern Biotech, 1:5,000), Goat Anti-Rabbit IgG(H+L) Human ads-HRP (Southern Biotech, 1:5000), Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3, 1:2000). .. Rabbit anti-K8.1 (gift of Denise Whitby, 1:10000) and Rabbit anti-SOX (Yenzyme, YZ6540 (cycle 1), 1:5000) were described previously.

    Article Title: Multi-proteomic profiling of the varicella-zoster virus-host interface reveals host susceptibilities to severe infection.
    Article Snippet: .. TFIIB (western blot (WB) 1:1,000; 4169; Cell Signaling Technology), MPP8 (WB 1:1,500; 16796; Proteintech), ZNF280D (WB 1:1,000; PA5-56410; Invitrogen), NPHP4 (WB 1:3,000; A8934; Abclonal), UBXN7 (IF 1:500; HPA049442; Sigma-Aldrich), IFI16 (WB 1:1,000, IF 1:500; 14970; Cell Signaling Technology), V5-tag rabbit (WB 1:1,000, IF 1:1,000; 13202; Cell Signaling Technology), V5-tag mouse (WB 1:1,000, IF 1:400; R960-25; Invitrogen), HA-tag (WB 1:2,500, IF 1:100; 2367; Cell Signaling Technology), HA-tag-HRP (WB 1:1,000; H6533; Sigma-Aldrich), β-actin-HRP (WB 1:2,500; sc-47778; Santa Cruz), β-tubulin (WB 1:500; 2128; Cell Signaling Technology). .. For WB, secondary antibodies conjugated to HRP detecting rabbit IgG (1:2,500) and mouse IgG (1:5,000) were purchased from Dako and Sigma-Aldrich, respectively.

    Article Title: Integrator enforces the fidelity of transcriptional termination at protein-coding genes
    Article Snippet: .. The antibodies used in this study are as follows: for ChIP-seq, RPB1 NTD (Cell Signaling Technology, 14958, lot #1), RPB1 Ser 2 (Abcam, 5095, lot #GR3225147-1), H3K36me3 (Abcam, 9050, lot #GR3257952-1), H3K36me2 (Abcam, 9049, lot #GR3236147-1), CSTF64 (Bethyl Laboratories, A301-92A, lot #A301-092A-2), and CPSF73 (Bethyl Laboratories, A301-019A, lot #A301-091A-1); for Western blot, INTS11 (Atlas Antibodies, HPA029025, lot #A107128), lamin A (Active Motif, 39961, lot #33310001), TFIIB (Cell Signaling Technology, 4169s, lot #1), CPSF100 (Bethyl Laboratories, A301-583A-M, lot #A301-583A-M-1), CSTF50 (Bethyl Laboratories, A301-250A-M, lot #A301-250A-M-3), CSTF64 (Bethyl Laboratories, A301-092A-M, lot #A301-092A-M-2), CPSF73 (Bethyl Laboratories, A301-091A-M, lot #A301-091A-M-1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, ab8245, lot #GR3317834-1). ..

    Saline:

    Article Title: The RNA polymerase II general transcription factor TFIIB is a target for transcriptome control during cellular stress and viral infection
    Article Snippet: 20-30 μg whole cell lysate was denatured in Laemmli Buffer (Bio-Rad), resolved by SDS-Page, and transferred to PVDF membrane (Bio-Rad) with a Trans-Blot Turbo (Bio-Rad, Mixed Molecular Weight protocol). .. Western blotting was performed with Tris-buffered saline and 0.2% Tween-20 (TBS-T) using PageRuler Prestained protein ladder (10-180 kDa formulation; ThermoFisher) and the following primary and secondary antibodies: TFIIB (clone 2F6A3H4, Cell Signaling-4169, 1:1000), GAPDH (anti-mouse, clone 6C5, Thermofisher-AM4300, 1:5000 or anti-rabbit, clone 14C10, Cell Signaling-2118, 1:1000), p27 (ProteinTech-15086-1-AP, 1:1000), TRIM28 (Cell Signaling-4123, 1:1000), TRIM24 (clone E93TN, Cell Signaling-79030, 1:1000), Vinculin (Abcam-ab91459, 1:1000), FK2 (Enzo-BML-PW8810-0100, 1:1000), NRF2 (ProteinTech-16396-1-AP, 1:000), Caspase-3 (Cell Signaling-9662, 1:1000), Caspase-8 (clone E7, Abcam-ab32397, 1:1000), γH2AX (Bethyl-A300-081A-M, 1:1000), p53 (clone 1C12, Cell Signaling-2524, 1:1000), Goat Anti-Mouse IgG(H+L) Human ads-HRP (Southern Biotech, 1:5,000), Goat Anti-Rabbit IgG(H+L) Human ads-HRP (Southern Biotech, 1:5000), Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3, 1:2000). .. Rabbit anti-K8.1 (gift of Denise Whitby, 1:10000) and Rabbit anti-SOX (Yenzyme, YZ6540 (cycle 1), 1:5000) were described previously.

    Formulation:

    Article Title: The RNA polymerase II general transcription factor TFIIB is a target for transcriptome control during cellular stress and viral infection
    Article Snippet: 20-30 μg whole cell lysate was denatured in Laemmli Buffer (Bio-Rad), resolved by SDS-Page, and transferred to PVDF membrane (Bio-Rad) with a Trans-Blot Turbo (Bio-Rad, Mixed Molecular Weight protocol). .. Western blotting was performed with Tris-buffered saline and 0.2% Tween-20 (TBS-T) using PageRuler Prestained protein ladder (10-180 kDa formulation; ThermoFisher) and the following primary and secondary antibodies: TFIIB (clone 2F6A3H4, Cell Signaling-4169, 1:1000), GAPDH (anti-mouse, clone 6C5, Thermofisher-AM4300, 1:5000 or anti-rabbit, clone 14C10, Cell Signaling-2118, 1:1000), p27 (ProteinTech-15086-1-AP, 1:1000), TRIM28 (Cell Signaling-4123, 1:1000), TRIM24 (clone E93TN, Cell Signaling-79030, 1:1000), Vinculin (Abcam-ab91459, 1:1000), FK2 (Enzo-BML-PW8810-0100, 1:1000), NRF2 (ProteinTech-16396-1-AP, 1:000), Caspase-3 (Cell Signaling-9662, 1:1000), Caspase-8 (clone E7, Abcam-ab32397, 1:1000), γH2AX (Bethyl-A300-081A-M, 1:1000), p53 (clone 1C12, Cell Signaling-2524, 1:1000), Goat Anti-Mouse IgG(H+L) Human ads-HRP (Southern Biotech, 1:5,000), Goat Anti-Rabbit IgG(H+L) Human ads-HRP (Southern Biotech, 1:5000), Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3, 1:2000). .. Rabbit anti-K8.1 (gift of Denise Whitby, 1:10000) and Rabbit anti-SOX (Yenzyme, YZ6540 (cycle 1), 1:5000) were described previously.

    Strep-tag:

    Article Title: The RNA polymerase II general transcription factor TFIIB is a target for transcriptome control during cellular stress and viral infection
    Article Snippet: 20-30 μg whole cell lysate was denatured in Laemmli Buffer (Bio-Rad), resolved by SDS-Page, and transferred to PVDF membrane (Bio-Rad) with a Trans-Blot Turbo (Bio-Rad, Mixed Molecular Weight protocol). .. Western blotting was performed with Tris-buffered saline and 0.2% Tween-20 (TBS-T) using PageRuler Prestained protein ladder (10-180 kDa formulation; ThermoFisher) and the following primary and secondary antibodies: TFIIB (clone 2F6A3H4, Cell Signaling-4169, 1:1000), GAPDH (anti-mouse, clone 6C5, Thermofisher-AM4300, 1:5000 or anti-rabbit, clone 14C10, Cell Signaling-2118, 1:1000), p27 (ProteinTech-15086-1-AP, 1:1000), TRIM28 (Cell Signaling-4123, 1:1000), TRIM24 (clone E93TN, Cell Signaling-79030, 1:1000), Vinculin (Abcam-ab91459, 1:1000), FK2 (Enzo-BML-PW8810-0100, 1:1000), NRF2 (ProteinTech-16396-1-AP, 1:000), Caspase-3 (Cell Signaling-9662, 1:1000), Caspase-8 (clone E7, Abcam-ab32397, 1:1000), γH2AX (Bethyl-A300-081A-M, 1:1000), p53 (clone 1C12, Cell Signaling-2524, 1:1000), Goat Anti-Mouse IgG(H+L) Human ads-HRP (Southern Biotech, 1:5,000), Goat Anti-Rabbit IgG(H+L) Human ads-HRP (Southern Biotech, 1:5000), Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3, 1:2000). .. Rabbit anti-K8.1 (gift of Denise Whitby, 1:10000) and Rabbit anti-SOX (Yenzyme, YZ6540 (cycle 1), 1:5000) were described previously.

    Chromatin Immunoprecipitation:

    Article Title: Integrator enforces the fidelity of transcriptional termination at protein-coding genes
    Article Snippet: .. The antibodies used in this study are as follows: for ChIP-seq, RPB1 NTD (Cell Signaling Technology, 14958, lot #1), RPB1 Ser 2 (Abcam, 5095, lot #GR3225147-1), H3K36me3 (Abcam, 9050, lot #GR3257952-1), H3K36me2 (Abcam, 9049, lot #GR3236147-1), CSTF64 (Bethyl Laboratories, A301-92A, lot #A301-092A-2), and CPSF73 (Bethyl Laboratories, A301-019A, lot #A301-091A-1); for Western blot, INTS11 (Atlas Antibodies, HPA029025, lot #A107128), lamin A (Active Motif, 39961, lot #33310001), TFIIB (Cell Signaling Technology, 4169s, lot #1), CPSF100 (Bethyl Laboratories, A301-583A-M, lot #A301-583A-M-1), CSTF50 (Bethyl Laboratories, A301-250A-M, lot #A301-250A-M-3), CSTF64 (Bethyl Laboratories, A301-092A-M, lot #A301-092A-M-2), CPSF73 (Bethyl Laboratories, A301-091A-M, lot #A301-091A-M-1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, ab8245, lot #GR3317834-1). ..



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